Regulatory

Part:BBa_K3431002:Design

Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang   Group: iGEM20_CSMU_Taiwan   (2020-07-21)


zp21_A Toehold Switch for miR-21 Detection


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We run thermodynamic tests for mRNA secondary structures with NUPACK and ViennaRNA to ensure its minimal free energy is in an appropriate range (lower than 0 kcal/mole)


Source

This is a synthetic sequence based upon Wang, S. et.al and Pardee, K. et al. for detection of miRNA-21. We obtained this sequence through custom gene synthesis of IDT.

References